MGB Eclipse® pairs a minor groove binding (MGB) tripeptide with a dark quencher. The MGB significantly enhances hybridization, while the Eclipse® has a broad absorption range that quenches many of the most common fluorophores, including FAM, HEX, TET and Yakima Yellow®.
Our line of fluorophore labels has expanded with the addition of new internal fluorophore-dT phosphoramidites, including JOE-dT, Quasar® 570-dT, and Quasar® 670-dT.
Since the DMT group binds strongly to reverse phase supports, the full-length sequences can be retained in a cartridge while the failure sequences are eluted.
2’-OMe-N6-Me-A, commonly referred to as m6Am, is a crucial component of the 5’-cap of mRNA. 2’-OMe-A is often the first transcribed nucleotide, and this modification is a result of methylation at the N6-position of the nucleobase. The 5’-cap structures initiate translation and protect RNA from degradation. Internal m6Am have also been implicated in regulating gene expression, RNA splicing, and cancer proliferation. Read more!
Explore Oligonucleotide Applications
Glen Research offers a wide range of products for oligonucleotide applications.
Glen Research offers a wide selection of fluorophores and fluorescence quenchers, which can be added during oligonucleotide synthesis at the 5′- or 3’-terminus or within the sequence. The resultant fluorescent oligonucleotide probes are typically used in a wide variety of applications like genetic and genome analyses, and forensic analysis. Common applications of fluorescent oligonucleotide probes are FRET and Molecular Beacon probes.